Agilent 1260 Fraction Collection System

The Acquity system comes complete with OQ Certification.

• The binary pump has been tested for faulty parts such as seals and check valves
• The detector has been tested for wavelength accuracy
• The autosampler has been tested for area of reproducibility
• The fraction collector diverter valve has been tested

This Agilent 1260 Infinity Analytical-scale fraction collection system is designed for fraction collection for flow rates between 100 µL/min and 10 mL/min.

We have combined a G1312B SL Binary pump, ALS injector, G1316A oven, G1314B VWD detector and G1379A degasser with the 1260 G1364C FC-AS (with a 900µL loop) to create a system perfect for peptide analysis:

Fraction collection features

• Lowest delay volumes and optimized tubing minimize peak dispersion and carryover between fractions
• Unique delay sensor makes it easy to determine delay volumes for accurate collection of fractions
• Various collection strategies enable pooling of fractions from multiple injections
• Multiple collection modes - with fraction triggering based on time, peak or mass - allow you to collect exactly the fractions you need
• System intelligence with CAN network processes data in real time for instantaneous and precise fraction collection
• GLP features such as Early Maintenance Feedback (EMF), electronic instrument logbook, IQ and OQ/PV, as well as a 21 CFR Part 11 compliant software solution, help you comply with quality and regulatory standards
• Forced fume extraction allows you to use the fraction collector outside a fume cupboard

Unique delay sensor

For accurate fraction collection you need to know the delay volume. A unique delay sensor makes determination of the delay volume an easy task. This delay sensor – comparable to a detector within the fraction collector – provides data to the Agilent ChemStation that calculates automatically the delay volume after the calibration measurement.

Multiple collection modes

The 1260 Infinity Fraction Collectors collect fractions automatically based on time, peak or mass. You can use any combination of time windows, thresholds, up and down slopes, upper thresholds and trigger sources such as UV or mass, or both. This allows you to collect exactly the fractions you need. The fraction preview function in the Agilent ChemStation software makes it easy to select the correct trigger parameters. Alternatively, you can use the 1200 Infinity Instant Pilot to collect fractions manually.

Various collection strategies

If a sample volume is too large for a single injection, you can do pooling by making repetitive injections from the same sample container and collecting the resulting fractions in the same fraction positions. Predefined recovery locations ensure that nothing gets lost. If, for any reason, an important compound was missed during fraction collection, it is not lost but collected in the recovery location that you assigned to the sample.

Fraction Collector Specifications

Trigger modes: Time slices and peak (threshold, up-/downslope, upper threshold and timetable), Boolean logic for different detector signals, combination of different modes, manual trigger (supported with 1200 Series Instant Pilot)

Trigger source: Agilent 1200 Series VWD (only for analytical scale), MWD and DAD detectors, Agilent 6100 Series Quadrupole LC/MS, third party detectors (require UIB) Diverter valve 3/2 valve, switching time < 100 ms

Maximum pressure 6 bar: Maximum capacity 3 fraction collectors connected in parallel through a 12-position/13-port selection valve.

Environment: 4 - 55 °C constant temperature, < 95% humidity (non-condensing)

System

• 1100 G1379A Solvent Degasser
• 1200 G1312B SL Binary Pump
• 1260 G1329B Autosampler
• 1260 G1316A Column Oven
• 1200 G1314B VWD
• 1260 G1364C FC-AS Fraction Collector
• PC with TFT screen
• Samsung Printer
• Chemstation LC 3D B.04.03 software

This system is in excellent working order (see video). Please contact us for a quotation or for rental terms.