Waters Acquity H-Class Binary UPLC System

H-Class System Specification

Dwell volume (total system): <400 µL (includes standard 100 µL mixer)

Integrated leak management: Leak sensors, as standard, and safe leak handling.

Quantum synchronization: Injection synchronization between pump and sample manager enhances retention time reproducibility.

pH range: 2 to 12

Unattended operation: Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software.

Cycle time: <30 s inject-to-inject

Settable flow rate range: 0.010 to 2.000 mL/min, in 0.001 mL increments (firmware version 1.5x and earlier).

Maximum operating pressure: 15,000 psi up to 1.0 mL/min,7800 psi up to 2.2 mL/min (firmware version 1.6x and later).

QSM Specification

Number of solvents: Blend up to four solvents in any combination (standard). Expanded solvent choices with optional six-port solvent select valve.

Solvent degassing: Integrated vacuum degassing, four chambers. One additional chamber for the SM-FTN purge solvent.

Solvent blending: Automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto•Blend Plus™ Technology.

Gradient formation: Low-pressure mixing, quaternary gradient.

Flow accuracy: ±1.0% at 0.5 to 2.0 mL/min using 100% A (with i² Valve). Back pressure 600 to 1000 psi with degassed H₂0.

SM-FTN Specification

Injection volume range: 0.1 to 10.0 µL as standard in 0.1 µL increments. Up to 1000.0 µL with optional extension loops.

Accuracy (aspiration): ±0.2 µL, measured by fluid weight removed from vial with 10.0 µL injections averaged over 20 injections using standard 100 µL syringe.

Linearity: >0.999 (standard needle) caffeine 0.030 mg/mL, ACN/H₂O (10:90), isocratic 0.6 mL/min, 0.2 to 10.0 µL, 1 to 70% needle volume.

Sample compartment: 4.0 to 40.0 °C, settable in 0.1 °C increments

PDA eλ Specification

Wavelength range 1: 190 to 800 nm

Wavelength accuracy: ±1 nm

Linearity range: Deviation at 2.0 AU ≤ 5%, propylparaben, at 257 nm

Data acquisition: Up to 80 Hz.

Pressure limit: 1000 psi

Flow cell design: Light-guiding UPLC intelligent technology flow cell.

CH-30A Specification

Column capacity: Single column, up to 4.6 mm internal diameter (I.D.), up to 300 mm lengthwith filter or guard column.

Column compartment temperature range: 20.0 to 90.0 °C, settable in 0.1 °C increments.

Column compartment temperature accuracy: ±0.5 °C at sensor.

Column compartment temperature stability: ±0.3 °C at sensor.

Solvent conditioning: Active pre-heating as standard.

Column tracking: eCord™ Technology column information management tracks and archives column usage history.

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